Journal
FERTILITY AND STERILITY
Volume 102, Issue 2, Pages 589-U349Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2014.05.012
Keywords
PCOS; ovary; insulin resistance; Chinese medicine; cryptotanshinone
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Funding
- National Clinical Trial Base in Chinese Medicine [JDZX2012036]
- Heilongjiang Province Universities [2011TD007]
- Project of Leading Excellent Innovative Talents of Heilongjiang University of Chinese Medicine
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Objective: To investigate the effects of cryptotanshinone (CRY), an active component of Chinese medicine, on ovarian androgen production, insulin resistance (IR), and glucose metabolism in mice. Design: Animal model and in vitro tissue model. Setting: University-affiliated laboratory. Animal(s): Mice. Intervention(s): Ovarian IR was induced by dexamethasone (DEX) in vivo. Animals were randomized to receive CRY treatment for 3 days or not. Ovulation rates, serum steroid levels, and glucose uptake in ovaries were quantified, and proteins in the phosphatidylinositol 3-hydroxy kinase pathway were measured. In vitro ovarian IR was also induced by DEX for 3 days. Ovarian steroid hormone secretion and glucose uptake were measured, and the hormone-synthesizing enzymes were determined by semiquantitative reverse transcription-polymerase chain reaction. Main Outcome Measure(s): Ovarian glucose uptake, in vivo ovulation rate, serum and culture medium steroid level, and molecular expression of phosphatidylinositol 3-hydroxy kinase and steroidogenic enzymes. Result(s): Dexamethasone significantly increased ovulation rates in vivo and increased T and E-2 production and decreased ovarian glucose uptake in vivo and in vitro. Cryptotanshinone significantly reduced ovulation rates in vivo and decreased T and estrogen production in vitro. Cryptotanshinone attenuated the inhibition of DEX on AKT2 and suppressed the up-regulation of CYP11 and CYP17 expression by DEX. Conclusion(s): Cryptotanshinone reversed DEX-induced androgen excess and ovarian IR in mice through activation of insulin signaling and the regulation of glucose transporters and hormone-synthesizing enzymes. This suggests a potential role for CRY in treating the ovulatory dysfunction associated with PCOS. (C) 2014 by American Society for Reproductive Medicine.
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