Journal
FERTILITY AND STERILITY
Volume 97, Issue 5, Pages 1234-+Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2012.02.032
Keywords
Female aging; ovarian function; oocyte quality; fertility; visfatin; VEGF
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Funding
- Ministry of Health and Welfare, Republic of Korea [A090602]
- Korea Health Promotion Institute [A090602] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Objective: To examine whether visfatin administration during superovulation improves ovarian response, developmental competence of oocytes, and fertility in aged female mice. Design: Controlled experimental study. Setting: University hospital. Animal(s): Two groups of differently aged C57BL female mice (6-11 and 26-31 weeks). Intervention(s): Female mice were coinjected intraperitoneally with 5 IU pregnant mare's serum gonadotropin (PMSG) and visfatin of various doses (0500 ng/mL), followed by 5 IU human chorionic gonadotropin (hCG) injection 48 hours later. Then the mice were immediately mated with an individual male. After 18 hours zygotes were cultured, and expression of ovarian visfatin and vascular endothelial growth factor (VEGF) was examined. Potential pregnancies of visfatin-administered aged female mice were monitored for delivery of offspring. Main Outcome Measure(s): Number of zygotes retrieved, embryo developmental competency, fertility potential, ovarian visfatin and VEGF expression. Result(s): Ovarian visfatin expression was significantly decreased in the aged mice group compared with the young. Visfatin administration significantly increased embryo developmental rate and ovarian visfatin and VEGF expressions in the aged mice. Visfatin-administered aged mice delivered significantly higher numbers of offspring than controls. Conclusion(s): This study suggests that visfatin administration during superovulation plays an important role in regulating oocyte quality and can improve oocyte quality and fertility of aged female mice. (Fertil Steril (R) 2012;97:1234-41. (C) 2012 by American Society for Reproductive Medicine.)
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