Journal
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
Volume 58, Issue 1, Pages 13-19Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
DOI: 10.1007/s13765-015-0003-4
Keywords
Flavonoid; AMPK; Kinase assay; Kaempferide; In silico docking
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Funding
- Korean Health Technology R&D project, Ministry of Health & Welfare, Republic of Korea [A111698]
- KU Research Professor Program of Konkuk University
- Korea Health Promotion Institute [A111698] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Eighteen flavonoids were tested as adenosine monophosphate-activated protein kinase ( AMPK) activators. The AMPK activation induced by kaempferide was 1.75 times better than the value obtained at 100 % AMPK phosphorylation. Western blotting analysis of kaempferide demonstrated that it increased the phosphorylation of AMPK in vitro. In silico docking results carried out to elucidate the binding mode between kaempferide and AMPK showed the docking pose of kaempferide in the binding site of AMPK was similar to that of the substrate contained in the crystallographic structure of AMPK. Flavonoids, including kaempferide, are promising potential AMPK activators.
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