Journal
FERTILITY AND STERILITY
Volume 93, Issue 1, Pages 159-166Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2008.09.038
Keywords
Cryopreservation; human sperm; DNA integrity; 8-oxoguanine; TUNEL assay; oxidative stress
Categories
Ask authors/readers for more resources
Objective: To evaluate the effect of cryopreservation on sperm motility and viability and to assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation before and after cryopreservation in liquid nitrogen. Design: Analysis of cryopreservation effects on sperm DNA integrity. Setting: Laboratory of Histology-Embryology of medicine faculty, Sfax, Tunisia. Patient(s): Fifteen semen samples from men undergoing infertility investigation. Intervention(s): Neat semen samples were cryopreserved in liquid nitrogen using a commercial freezing medium (SpermFreeze, Fertipro, Belgium) according to the manufacturer's instructions. Samples were thawed at room temperature. Main Outcome Measure(s): Sperm DNA fragmentation was assessed using terminal deoxynucteotidyl transferase (Tdt) mediated dUTP nick end labeling and sperm DNA oxidation was determined using a fluorescent assay (Oxy-DNA test) for the detection of 8-oxoguanine. Evaluation of DNA fragmentation and oxidation rates was carried out before and after cryopreservation using flow cytometry. Result(s): A significant decrease in sperm motility and viability was observed after cryostorage. In addition, sperm DNA fragmentation and DNA oxidative damage increased significantly after cryopreservation/thaw. Conclusion(s): Cryopreservation has deleterious effects on sperm DNA by inducing DNA fragmentation and oxidation but the mechanisms underlying such damages need to be elucidated by further investigations. (Fertil Steril(R) 20 10;93:159-66. (C)2010 by American Society for Reproductive Medicine.)
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available