Journal
FERTILITY AND STERILITY
Volume 93, Issue 8, Pages 2486-2490Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2009.06.015
Keywords
Sperm DNA damage; preimplantation embryos; genomic instability; transgenerational effect
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Funding
- Indian Council of Medical Research [2006-01640]
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Objective: To report a more quantitative approach to study the influence of varying levels of sperm DNA damage on transgenerational changes in genomic instability in a mouse model. Design: Experimental prospective study. Setting: Embryology research laboratory. Animal(s): Swiss albino mice. Intervention(s): The sperm DNA damage was induced by different doses of g-irradiation to male mice followed by mating with healthy female mice. Main Outcome Measure(s): Genomic integrity in embryos, fetus, and spermatozoa of F1 mice derived from the DNA-damaged sperm. Result(s): The transgenerational changes in genetic integrity were attributed by a dose-dependent increase in the frequency of micronuclei in preimplantation embryos and a concomitant increase in genomic instability in fetal liver cells and sperm chromatin modifications in F1 males. A strong positive correlation was observed between the extent of sperm DNA damage and somatic and germ-line genomic instability. Conclusion(s): Sperm-mediated transgenerational genomic instability is dependent on the amount of DNA damage present in the sire's sperm at the time of fertilization. (Fertil Steril (R) 2010; 93: 2486-90. (C) 2010 by American Society for Reproductive Medicine.)
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