Journal
FERTILITY AND STERILITY
Volume 91, Issue 2, Pages 589-596Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2007.11.067
Keywords
L-Carnitine; antioxidant; actinomycin-D; blastocyst development rate; TNF-alpha
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Objective: To optimize the L-carnitine (LC) concentration as a supplement in embryo culture medium and to investigate the effect of LC on developing embryos. Design: Experimental study. Setting: Reproductive research center at a tertiary hospital. Intervention(S): To optimize the LC concentration, 420 mouse embryos were divided into seven groups and incubated with different LC concentrations (0, 0.3, 0.6, 1.2, 2.5, 5.0, and 10 mg/mL). To investigate the effect of LC on the developing embryos, 500 mouse embryos were divided into three groups and incubated with either actinomycin-D (AD; 0.005 mu g/mL), hydrogen peroxide (H2O2; 500 mu mol/L), or tumor necrosis factor alpha (TNF-alpha; 500ng) with and without LC 0.3 or 0.6 mg/mL. Blastocyst development rate (%BDR) and DNA damage were examined for all groups. Main Outcome Measure(s): Effect of LC on embryogenesis. Result(s): Significant improvement in %BDR was seen at LC 0.3 mg/mL compared with the control (p = 0.006). L-Carnitine at 0.3 and 0.6 mg/mL significantly reduced the blocking effect of AD, H2O2, and TNF-alpha and significantly decreased the level of DNA damage. Conclusion(S): Embryo culture medium supplementation with LC may offer a novel and a cost-effective technique to improve the embryogenesis of cultured embryos. This may be beneficial in improving IVF outcomes. (Fertil Steril(R) 2009;91:589-96. (C)2009 by American Society for Reproductive Medicine.)
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