Anti-Mullerian hormone substance from follicular fluid is positively associated with success in oocyte fertilization during in vitro fertilization

Objective: To determine whether the anti-Mullerian hormone (AMH) level in follicular fluid is associated with success in oocyte fertilization during in vitro fertilization (IVF) cycles. Design: Retrospective study. Setting: Tokyo Medical University hospital. Patient(s): Thirty-one women undergoing IVF cycles. Intervention(s): The women were divided into two groups according to the success or failure of fertilization. Follicular fluid samples were obtained from a single follicle in each patient. Main Outcome Measure(s): Assessment of values for AMH, inhibin B, and estradiol from follicular fluid and serum on the day of oocyte retrieval during IVF treatment. Result(s): The follicular fluid AMH levels from fertilized patients (group 1) were 3.42 times higher than from non-fertilized (group 2). There was no correlation between the ratio of the high quality grade embryos and either serum AMH or inhibin B levels. There was no statistically, significant correlation between serum AMH and the number of oocytes. However, there was a strong correlation between levels of serum estradiol, inhibin B, and the number of oocytes. Conclusion(s): Oocytes are more likely to be fertilized when follicles are able to make high concentrations of AMH in the follicular fluid. Thus, AMH could be a prediction marker for fertilization.