Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study

Objective: To evaluate the effect of vaginal lubricants Pre similar to Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity. Design: Prospective, comparative, in vitro study. Setting: Andrology laboratory at tertiary care hospital. Patient(s): Thirteen normozoospermic donors. Intervention(s): Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre similar to Seed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre similar to Seed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay. Main Outcome Measure(s): Sperm motility and percent DNA fragmentation index. Results: Percent motility did not differ significantly between HTF controls and Pre similar to Seed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre similar to Seed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly. Conclusion: Pre similar to Seed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples. (Fertil Steril (R) 2008;89:375-9. (c) 2008 by American Society for Reproductive Medicine.)