Journal
FEMS YEAST RESEARCH
Volume 13, Issue 2, Pages 241-244Publisher
WILEY-BLACKWELL
DOI: 10.1111/1567-1364.12028
Keywords
biofuel; lignocellulose; xylose; pentose; isobutanol; metabolic engineering
Funding
- Butalco GmbH, Switzerland
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Simultaneous overexpression of an optimized, cytosolically localized valine biosynthesis pathway together with overexpression of xylose isomerase XylA from Clostridium phytofermentans, transaldolase Tal1 and xylulokinase Xks1 enabled recombinant Saccharomyces cerevisiae cells to complement the valine auxotrophy of ilv2,3,5 triple deletion mutants for growth on d-xylose as the sole carbon source. Moreover, after additional overexpression of ketoacid decarboxylase Aro10 and alcohol dehydrogenase Adh2, the cells were able to ferment D-xylose directly to isobutanol.
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