Journal
FEMS YEAST RESEARCH
Volume 12, Issue 3, Pages 293-304Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1567-1364.2011.00770.x
Keywords
Saccharomyces cerevisiae; cell morphology; CalMorph; bud elongation; actin; principal components analysis
Funding
- New Energy and Industrial Technology Development Organization (NEDO)
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Grants-in-Aid for Scientific Research [20380063, 19057002, 11J06399] Funding Source: KAKEN
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To investigate the biological activity of a novel 24-membered macrolide compound, JBIR-19, isolated from the culture broth of the entomopathogenic fungus Metarhizium sp. fE61, morphological changes in yeast cells were examined using the automated image-processing program CalMorph. Principal components analysis was used to elucidate dynamic changes in the phenotypes, revealing two independent effects of JBIR-19 in yeast cells: bud elongation and increased size of the actin region. Using a fitness assay, we identified the genes required for robust growth in the presence of JBIR-19. Among these were CCW12, YLR111W, and DHH1, which are also involved in abnormal bud morphology. Based on these results and others, we predict intracellular targets of JBIR-19 and its functional interactions.
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