Journal
FEMS YEAST RESEARCH
Volume 11, Issue 6, Pages 524-527Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1567-1364.2011.00737.x
Keywords
Arv1; yeast; membrane topology
Funding
- Medical Diagnostic Laboratories, L.L.C.
Ask authors/readers for more resources
In Saccharomyces cerevisiae, ARV1 encodes a 321 amino acid transmembrane protein localized to the endoplasmic reticulum (ER) and Golgi. It has been shown previously that arv1 cells harbor defects in sphingolipid and glycosylphosphatidylinositol biosyntheses, and may harbor sterol trafficking defects. Using C-terminal fusion to Suc2-His4, we determined the orientation of full-length Arv1 in the ER membrane. Once membrane topology was determined, we used this information and truncation analysis to establish the minimum protein length required for Arv1 function and phenotypic suppression. By understanding the topology of Arv1 we can now further analyze its putative lipid and glycosylphosphatidylinositol intermediate transport activities.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available