Journal
FEMS YEAST RESEARCH
Volume 9, Issue 8, Pages 1217-1225Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1567-1364.2009.00583.x
Keywords
Saccharomyces cerevisiae; Saccharomyces paradoxus; haploid; barcode; resequencing; strains
Funding
- The Wellcome Trust and Biotechnology and Biological Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/F015216/1] Funding Source: researchfish
- BBSRC [BB/F015216/1] Funding Source: UKRI
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Saccharomyces cerevisiae has proved to be an invaluable model in classical and molecular genetics studies. Despite several hundreds of isolates already available, the scientific community relies on the use of only a handful of unrelated strains. The lack of sequence information, haploid derivatives and genetic markers has prevented novel strains from being used. Here, we release a set of 55 S. cerevisiae and Saccharomyces paradoxus genetically tractable strains, previously sequenced in the Saccharomyces Genome Resequencing Project. These strains are stable haploid derivatives and ura3 auxotrophs tagged with a 6-bp barcode, recognized by a restriction enzyme to allow easy identification. We show that the specific barcode can be used to accurately measure the prevalence of different strains during competition experiments. These strains are now amenable to a wide variety of genetic experiments and can be easily crossed with each other to create hybrids and segregants, providing a valuable resource for breeding programmes and quantitative genetic studies. Three versions of each strain (haploid Mat a and Mat a and diploid Mat a/alpha all as ura3::KanMX-Barcode) are available through the National Culture Yeast Collection.
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