Journal
FEMS MICROBIOLOGY LETTERS
Volume 357, Issue 2, Pages 136-143Publisher
OXFORD UNIV PRESS
DOI: 10.1111/1574-6968.12530
Keywords
environmental stress; antibiotic resistance; gene expression
Categories
Funding
- Natural Science and Engineering Research Council
- University of Manitoba
Ask authors/readers for more resources
In this study, we examined the impact of various environmental conditions on the expression of resistance-nodulation-division (RND) efflux pumps and outer membrane (OM) porins, two key determinants of Acinetobacter baumannii's intrinsic resistance, an organism known to cause various multidrug resistant infections in immunocompromised individuals. Quantitative RT-PCR was used to analyze the expression of adeB, adeG, and adeJ (genes encoding RND pumps) and 33 kDa, carO, and oprD (genes encoding OM porins) of A. baumannii ATCC19606(T) under different incubation temperatures (30, 37, and 42 degrees C) and in the presence of high osmolarity and salicylate. Downregulation of all three RND pumps was observed at 30 degrees C, while downregulation of all three porins tested was observed at increased osmolarity. Downregulation of RND efflux pumps, particularly AdeABC, was consistent with increased susceptibility to antibiotics that are substrates of this pump. Expression of the adeR response regulator gene of the AdeRS system, the activator of the AdeABC pump, was also analyzed. Our work shows that various environmental stress conditions can influence the expression of RND pumps and porins in A. baumannii ATCC19606(T) and thus may play a role in the modulation of its antibiotic resistance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available