Journal
FEMS MICROBIOLOGY LETTERS
Volume 356, Issue 1, Pages 89-96Publisher
OXFORD UNIV PRESS
DOI: 10.1111/1574-6968.12483
Keywords
antifungal activity; lactate dehydrogenase; Lactobacillus plantarum; phenyl lactic acid
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Funding
- National Natural Science Foundation of China [30900961, 20876116]
- Tianjin University of Science and Technology Research Fund Project [20130111]
- Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University
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Phenyl lactic acid (PLA) has been widely reported as a new natural antimicrobial compound. In this study, 120 Lactobacillus plantarum strains were demonstrated to produce PLA using high-performance liquid chromatography. Lactobacillus plantarum IMAU10124 was screened with a PLA yield of 0.229 g L-1. Compared with all previous reports, this is the highest PLA-producing lactic acid bacteria (LAB) when grown in MRS broth without any optimizing conditions. When 3.0 g L-1 phenyl pyruvic acid (PPA) was added to the medium as substrate, PLA production reached 2.90 g L-1, with the highest 96.05% conversion rate. A lowest PLA-yielding L. plantarum IMAU40105 (0.043 g L-1) was also screened. It was shown that the conversion from PPA to PLA by lactic dehydrogenase (LDH) is the key factor in the improvement of PLA production by LAB. Comparing the LDH gene of two strains, four amino acid mutation sites were found in this study in the LDH of L. plantarum IMAU10124.
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