Journal
FEMS MICROBIOLOGY LETTERS
Volume 334, Issue 1, Pages 22-26Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2012.02613.x
Keywords
Aeromonas hydrophila; captive snakes; bacteraemia; temperature stress; 16S rRNA gene sequencing
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Captive snakes, that is, a Jamaican boa (Epicrates subflavus) a yellow anaconda (Eunectes notaeus) and a corn snake (Pantherophis guttatus guttatus), died with signs of bacteraemia including the presence of petechial haemorrhages in the mouth and gums and haemorrhages in the lung, spleen and intestines. The abdomen and anus were swollen with bloody-tinged mucus in the colon. Aeromonas hydrophila was recovered in dense virtually pure culture growth from the internal organs. Characterization of the isolates was by phenotyping and sequencing of the 16S rRNA gene (sequence homology of 99% with A.similar to hydrophila) with outputs confirming the identity as A.similar to hydrophila. Pathogenicity experiments confirmed virulence to frogs (Rana esculenta) and rainbow trout (Oncorhynchus mykiss).
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