Journal
FEMS MICROBIOLOGY LETTERS
Volume 330, Issue 1, Pages 17-22Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2012.02515.x
Keywords
Apis mellifera; Ascosphaera; chalkbrood; DNA sequences; entomopathogenic fungi
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Funding
- Danish National Research Foundation
- Danish Council for Strategic Research
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The pathogenic fungus Ascosphaera apis is ubiquitous in honey bee populations. We used the draft genome assembly of this pathogen to search for polymorphic intergenic loci that could be used to differentiate haplotypes. Primers were developed for five such loci, and the species specificities were verified using DNA from nine closely related species. The sequence variation was compared among 12 A.apis isolates at each of these loci, and two additional loci, the internal transcribed spacer of the ribosomal RNA (ITS) and a variable part of the elongation factor 1a (Ef1a). The degree of variation was then compared among the different loci, and three were found to have the greatest detection power for identifying A.apis haplotypes. The described loci can help to resolve strain differences and population genetic structures, to elucidate hostpathogen interaction and to test evolutionary hypotheses for the world's most important pollinator: the honey bee and one of its most common pathogens.
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