Journal
FEMS MICROBIOLOGY LETTERS
Volume 322, Issue 2, Pages 108-114Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1574-6968.2011.02337.x
Keywords
Novosphingobium; degradation efficiency; gene expression; frame-shift mutation
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Funding
- State Key Basic Research and Development Plan of China [2008CB418002]
- National Water Science and Technology Projects [2009ZX07101-013-02]
- Chinese Academy of Sciences [082303-1-501]
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The microcystin-degrading genes, mlr, are important participants in the degradation process of hepatotoxic microcystins for several bacterial species. However, their expression status during degrading microcystins is still unknown. In order to study this expression process, we isolated a novel microcystin-degrading bacterial strain, sequenced its mlr gene cluster and examined the expression of the mlrA gene at different concentrations of microcystin LR. The expression of mlrA increased slightly at 0.4 mg L(-1), and was significantly upregulated at 2.0 mg L(-1). Frameshift mutations were found in the mlrB* gene, and the mRNA of mlrB* could not be detected in the total RNA extracts of Novosphingobium sp. THN1. We conclude that mlrA is actively involved in the microcystin-degrading process, but mlrB* has lost its activity in this bacterial strain.
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