Journal
FEMS MICROBIOLOGY LETTERS
Volume 306, Issue 1, Pages 72-81Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2010.01938.x
Keywords
Photobacterium; Aliivibrio; luciferase; autoinduction; symbiosis
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Funding
- W.M. Keck Foundation
- University of Georgia Graduate Research Fellowship
- National Defense Science and Engineering Graduate Fellowship
- National Science Foundation [MCB-0347317]
- National Institutes of Health [RO1 A150661]
- Army Research Office [49549LSII]
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Vibrio fischeri induces both anaerobic respiration and bioluminescence during symbiotic infection. In many bacteria, the oxygen-sensitive regulator FNR activates anaerobic respiration, and a preliminary study using the light-generating lux genes from V. fischeri MJ1 cloned in Escherichia coli suggested that FNR stimulates bioluminescence. To test for FNR-mediated regulation of bioluminescence and anaerobic respiration in V. fischeri, we generated fnr mutants of V. fischeri strains MJ1 and ES114. In both strains, FNR was required for normal fumarate- and nitrate-dependent respiration. However, contrary to the report in transgenic E. coli, FNR mediated the repression of lux. ArcA represses bioluminescence, and P-arcA-lacZ reporters showed reduced expression in fnr mutants, suggesting a possible indirect effect of FNR on bioluminescence via arcA. Finally, the fnr mutant of ES114 was not impaired in colonization of its host squid, Euprymna scolopes. This study extends the characterization of FNR to the Vibrionaceae and underscores the importance of studying lux regulation in its native background.
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