Journal
FEMS MICROBIOLOGY LETTERS
Volume 278, Issue 1, Pages 86-93Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2007.00979.x
Keywords
Francisella tularensis; novicida; tularemia; counter-selection; plasmid
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI056215] Funding Source: NIH RePORTER
- NIAID NIH HHS [5R01 AI056215-02] Funding Source: Medline
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Francisella novicida is a gram-negative pathogen that can induce disease in mice that mimics human tularemia, and is nearly identical to Francisella tularensis at the genomic level. In this work a number of antibiotic marker cassettes that incorporate a strong F. novicida promoter is constructed, which greatly enhances selection in F. novicida and F. tularensis. Two low-copy plasmid vectors based on a broad-host-range plasmid, and an integrating vector have also been made, and these can be used for genetic complementation. Two general approaches to deletion mutagenesis in F. novicida is also described.
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