Journal
FEMS MICROBIOLOGY LETTERS
Volume 288, Issue 2, Pages 235-240Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6968.2008.01353.x
Keywords
isobutyronitrile; soda solonchak soils; haloalkaliphilic; Bacillus
Categories
Funding
- NWO-RFBR [047.011.2004.010]
- RFBR [07-04-00153]
- Russian Academy of Sciences
- Delft Research Center of Life Sciences and Technology (DRC-LST)
Ask authors/readers for more resources
Enrichment with isobutyronitrile as the sole carbon, energy and nitrogen source at pH 10, using soda solonchak soils as an inoculum, resulted in the selection of a binary culture consisting of two different spore-forming phenotypes. One of them, strain ANL-iso4, was capable of growth with isobutyronitrile as a single substrate, while the other phenotype only utilized products of isobutyronitrile hydrolysis, such as isobutyroamide and isobutyrate. Strain ANL-iso4 is an obligate alkaliphile and a moderately salt-tolerant bacterium. Apart from isobutyronitrile, it grew on other (C3-C6) aliphatic nitriles at pH 10. Resting cells of ANL-iso4 actively hydrolyzed a number of aliphatic and arylaliphatic nitriles and their corresponding amides. The latter, together with the intermediate formation of amides during nitrile hydrolysis, indicated the presence of a nitrile hydratase/amidase system in the novel bacterium. Although present in an alkaliphilic bacterium, both nitrile- and amide-hydrolyzing activities had a pH optimum within the neutral range, probably due to their intracellular localization. On the basis of phenotypic and phylogenetic analyses, strain ANL-iso4 is proposed as a new species Bacillus alkalinitrilicus sp. nov.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available