Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 82, Issue 2, Pages 501-509Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2012.01442.x
Keywords
permafrost; mycelial fungi; biodiversity; ITS2
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Funding
- Russian Foundation for Basic Research [09-04-01251-a]
- MCB of the Russian Academy of Sciences
- Ministry of Education and Science of the Russian Federation [16.518.11.7035]
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Filamentous fungi in 36 samples of Antarctic permafrost sediments were studied. The samples collected during the Russian Antarctic expedition of 20072009 within the framework of the Antarctic Permafrost Age Project (ANTPAGE) were recovered from different depths in ice-free oases located along the perimeter of the continent. Fungal diversity was determined by conventional microbiological techniques combined with a culture-independent method based on the analysis of internal transcribed spacer (ITS2) sequences in total DNA of the samples. The study revealed a rather low fungal population density in permafrost, although the diversity found was appreciable, representing more than 26 genera. Comparison of the data obtained by different techniques showed that the culture-independent method enabled the detection of ascomycetous and basidiomycetous fungi not found by culturing. The molecular method failed to detect members of the genera Penicillium and Cladosporium that possess small-sized spores known to have a high resistance to environmental changes.
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