Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 74, Issue 1, Pages 136-145Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2010.00942.x
Keywords
Methylobacterium extorquens; qRT-PCR; monitoring; strawberry leaves
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Funding
- Austrian Research Promotion Agency (FFG)
- Grunewald Fruchtsaft GmbH
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Besides its influence on plant growth and health, plant-associated bacteria exert an impact on fruit quality. Methylotrophic bacteria can enhance the biosynthesis of strawberry flavor compounds, especially the two furanoid compounds 2,5-dimethyl-4-hydroxy-2H-furanone (DMHF) and 2,5-dimethyl-4-methoxy-2H-furanone in vitro. Here, we report the selection and characterization of Methylobacterium extorquens DSM 21961, a strain that was able to enhance the furanone content ad planta under greenhouse conditions. For monitoring the colonization of strawberry plants, a strain-specific quantification system for M. extorquens DSM 21961 was developed. Specificity, linear range and quantitative limit of the system were shown, and successful application was demonstrated in a monitoring experiment of M. extorquens DSM 21961 on strawberry leaves under greenhouse conditions. Furthermore, the quantification of DMHF in strawberry fruits via GC indicated an increased biosynthesis of this compound in strawberry plants. The colonization behavior analyzed by confocal laser scanning microscopy using GFP-tagged cells revealed high colonization of the upper and the lower leaf surfaces, with a specific accumulation of bacterial cells on trichomes. The results support a biotechnological application of this promising flavor-stimulating agent.
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