Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 65, Issue 1, Pages 125-132Publisher
OXFORD UNIV PRESS
DOI: 10.1111/j.1574-6941.2008.00497.x
Keywords
methane oxidation; nitrogen fertilization; urea; stable isotope probing; Methylomicrobium; Methylocaldum
Categories
Ask authors/readers for more resources
Methane-oxidizing bacteria (MOB) in soil are not only controlled by their main substrates, methane and oxygen, but also by nitrogen availability. We compared an unfertilized control with a urea-fertilized treatment and applied RNA-stable-isotope-probing to follow activity changes upon fertilization as closely as possible. Nitrogen fertilization of an Italian rice field soil increased the CH4 oxidation rates sevenfold. In the fertilized treatment, isopycnic separation of C-13-enriched RNA became possible after 7 days when 300 mu mol (CH4)-C-13 g(dry soil)(-1) had been consumed. Terminal-restriction fragment length polymorphism (T-RFLP) fingerprints and clone libraries documented that the type I methanotrophic genera Methylomicrobium and Methylocaldum assimilated (CH4)-C-13 nearly exclusively. Although previous studies had shown that the same soil contains a much larger diversity of MOB, including both type I and type II, nitrogen fertilization apparently activated only a small subset of the overall diversity of MOB, type I MOB in particular.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available