Competition of Fe(III) reduction and methanogenesis in an acidic fen

Peatlands are sources of relevant greenhouse gases such as CH4, but the temporal presence of Fe(III) may inhibit methanogenesis. Because excess of carbon during the vegetation period might allow concomitant electron-accepting processes, Fe(III) reduction and methanogenesis were studied during an annual season in an acidic fen. The upper peat layer displayed the highest Fe(II)- and CH4-forming activities. The rates of Fe(II) formation did not change during the year and methanogenesis started mostly when Fe(II) formation reached a plateau. Most of the Fe(III) pool seemed to be bioavailable, and addition of nitrilotriacetic acid stimulated only light Fe(II) formation, whereas EDTA and anthraquinone-2,6-disulfonate had no effect. In the presence of an inhibitor for methanogenesis (sodium 2-bromoethanesulfonate), Fe(II) formation was inhibited to 45%. Addition of Fe(III) during ongoing methanogenesis led only to a partial inhibition of CH4 formation. The proportion of acetoclastic methanogenesis varied between 42% and 90%, but no trend with time was observed. The number of acetate-, ethanol- or lactate-utilizing Fe(III) reducers approximated 10(5)-10(6) cells g (fresh wt peat)(-1). Fermentative glucose-utilizing Fe(III)-reducers were most abundant. Our results suggest that (1) methanogens used Fe(III) as an electron acceptor and (2) fermenting bacteria, which do not compete with methanogens for common electron donors, dominated the reduction of Fe(III) in this fen.