Journal
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
Volume 54, Issue 2, Pages 215-223Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1574-695X.2008.00466.x
Keywords
Lactobacillus plantarum; host-microorganism interaction; PAP; RegIII; msa; srtA; gene expression; probiotic
Categories
Funding
- Centre for Human Nutrigenomics
- The Netherlands
- EADGENE [FOOD-CT-2004-506416]
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Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8 h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
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