Journal
FEBS LETTERS
Volume 592, Issue 18, Pages 3062-3073Publisher
WILEY
DOI: 10.1002/1873-3468.13222
Keywords
Arabidopsis; chlorophyll; chlorophyll synthase; cyanobacteria; high light-inducible proteins; YidC/Alb3/OxaI
Funding
- University of Sheffield Faculty of Science Studentship
- European Research Council [338895]
- Biotechnology and Biological Sciences Research Council (UK) [BB/M000265/1]
- Ministry of Education of the Czech Republic [LO1416]
- Czech Science Foundation [17-08755S]
- BBSRC [BB/M000265/1] Funding Source: UKRI
- European Research Council (ERC) [338895] Funding Source: European Research Council (ERC)
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In the model cyanobacterium Synechocystis sp. PCC 6803, the terminal enzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms a complex with high light-inducible proteins, the photosystem II assembly factor Ycf39 and the YidC/Alb3/Oxal membrane insertase, co-ordinating chlorophyll delivery with cotranslational insertion of nascent photosystem polypeptides into the membrane. To gain insight into the ubiquity of this assembly complex in higher photosynthetic organisms, we produced functional foreign chlorophyll syntheses in a cyanobacterial host. Synthesis of algal and plant chlorophyll syntheses allowed deletion of the otherwise essential native cyanobacterial gene. Analysis of purified protein complexes shows that the interaction with YidC is maintained for both eukaryotic enzymes, indicating that a ChlG-YidC/Alb3 complex may be evolutionarily conserved in algae and plants.
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