Journal
FEBS LETTERS
Volume 588, Issue 18, Pages 3435-3440Publisher
WILEY
DOI: 10.1016/j.febslet.2014.07.036
Keywords
Lytic polysaccharide monooxygenase; AA10; AA9; AA11; GbpA; Colonization factor; Vibrio cholerae
Funding
- Norwegian Research Council [214138, 214613, 216162]
- Vista program of the Norwegian Academy of Science and Letters [6505]
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The discovery of the copper-dependent lytic polysaccharide monooxygenases (LPMOs) has revealed new territory for chemical and biochemical analysis. These unique mononuclear copper enzymes are abundant, suggesting functional diversity beyond their established roles in the depolymerization of biomass polysaccharides. At the same time basic biochemical methods for characterizing LPMOs, such as activity assays are not well developed. Here we describe a method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity. The method was used to quantify the activity of a four-domain LPMO from Vibrio cholerae, GbpA, which is a virulence factor with no obvious role in biomass processing. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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