Journal
FEBS LETTERS
Volume 588, Issue 19, Pages 3603-3612Publisher
WILEY
DOI: 10.1016/j.febslet.2014.06.043
Keywords
Super-resolution imaging; Fluorophore; Photoswitch
Funding
- University of Washington
- Burroughs-Wellcome Career Award at the Scientific Interface
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Photoswitchable fluorescent probes are key elements of newly developed super-resolution fluorescence microscopy techniques that enable far-field interrogation of biological systems with a resolution of 50 nm or better. In contrast to most conventional fluorescence imaging techniques, the performance achievable by most super-resolution techniques is critically impacted by the photoswitching properties of the fluorophores. Here we review photoswitchable fluorophores for super-resolution imaging with discussion of the fundamental principles involved, a focus on practical implementation with available tools, and an outlook on future directions. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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