Journal
FEBS LETTERS
Volume 588, Issue 9, Pages 1590-1595Publisher
WILEY
DOI: 10.1016/j.febslet.2014.02.046
Keywords
Photosensitizer; Gramicidin channel; Bilayer lipid membrane; Unsaturated lipid; Singlet oxygen; Adsorption
Funding
- Russian Foundation for Basic Research [12-04-00199]
- Lomonosov Moscow State University Development Programme
- Ministry of Education and Science of the Russian Federation
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In contrast to expectations that unsaturated fatty acids contribute to oxidative stress by providing a source of lipid peroxides, we demonstrated the protective effect of double bonds in lipids on oxidative damage to membrane proteins. Photodynamic inactivation of gramicidin channels was decreased in unsaturated lipid compared to saturated lipid bilayers. By estimating photosensitizer (boronated chlorine e(6) amide) binding to the membrane with the current relaxation technique, the decrease in gramicidin photoinactivation was attributed to singlet oxygen scavenging by double bonds in lipids rather than to the reduction in photosensitizer binding. Gramicidin protection by unsaturated lipids was also observed upon induction of oxidative stress with tert-butyl hydroperoxide. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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