Journal
FEBS LETTERS
Volume 588, Issue 14, Pages 2344-2352Publisher
WILEY
DOI: 10.1016/j.febslet.2014.05.033
Keywords
Osteoarthritis; MicroRNA; MicroRNA-145; Interleukin-1 beta; Extracellular matrix
Funding
- Nature Science Foundation of China [81201431]
- National Basic Research Program [2011CB964701]
- General Program of Nature Science Foundation of China [NO81271980]
- National Key Technology Research and Development Program of China [2012BAI42G01]
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MicroRNA-145 has been shown to regulate chondrocyte homeostasis. It seems that miR-145 is implicated in cartilage dysfunction in Osteoarthritis (OA). However, the functional role of miR-145 in interleukin-1 beta (IL-1 beta)-induced extracellular matrix (ECM) degradation of OA cartilage has never been clarified. Here, we show that miR-145 expression increased in OA chondrocytes and in response to IL-1 beta stimulation. We confirm that mothers against decapentaplegic homolog 3 (Smad3), a key factor in maintaining chondrocyte homeostasis, is directly regulated by miR-145. Modulation of miR-145 affects the expression of Smad3 causing a change of its downstream target gene expression as well as IL-1 beta-induced ECM degradation in OA chondrocytes. This indicates that miR-145 contributes to impaired ECM in OA cartilage probably in part via targeting Smad3. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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