Journal
FEBS LETTERS
Volume 588, Issue 23, Pages 4497-4503Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.10.021
Keywords
Pig; MiR-92a; Smad7; Granulosa cell apoptosis; RNA interference
Funding
- Fundamental Research Funds for the Central Universities [KYZ201152]
- National Key Scientific Program Grant [2014CB138502]
- National Natural Science Foundation of China [30901027]
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Smad7 has a key role in apoptosis of mammalian ovarian granulosa cells (GCs), as it antagonizes and fine-tunes transforming growth factor beta (TGF beta) signaling. This study demonstrates that miR-92a regulates GC apoptosis in pig ovaries by targeting Smad7 directly. The expression level of miR-92a was down-regulated in atretic porcine follicles, whereas miR-92a expression led to inhibition of GC apoptosis. The Smad7 gene was identified as a direct target of miR-92a using a dual-luciferase reporter assay. Transfection of GCs with miR-92a mimics decreased Smad7 mRNA and protein levels, whereas expression of an miR-92a inhibitor in GCs had the opposite effect. In addition, knockdown of Smad7 prevented GC apoptosis in cells that expressed the miR-92a inhibitor. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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