Journal
FEBS LETTERS
Volume 588, Issue 18, Pages 3417-3426Publisher
WILEY
DOI: 10.1016/j.febslet.2014.07.034
Keywords
Type-3 copper; Polyphenol oxidase; Aurone synthase; Heterologous expression; 4-Deoxyaurone; Coreopsis grandiflora
Funding
- Austrian Science Fund (FWF) [P25217-N28]
- graduate training program Functional Molecules [IK I041-N]
- Deutsche Forschungsgemeinschaft (DFG) [Ro 1084/8-1]
- FWF [P24331-B16]
- Austrian Science Fund (FWF) [P 25217, P 24331] Funding Source: researchfish
- Austrian Science Fund (FWF) [P24331] Funding Source: Austrian Science Fund (FWF)
Ask authors/readers for more resources
Polyphenol oxidases are involved in aurone biosynthesis but the gene responsible for 4-deoxyaurone formation in Asteraceae was so far unknown. Three novel full-length cDNA sequences were isolated from Coreopsis grandiflora with sizes of 1.80 kb (cgAUS1) and 1.85 kb (cgAUS2a, 2b), encoding for proteins of 68-69 kDa, respectively. cgAUS1 is preferably expressed in young petals indicating a specific role in pigment formation. The 58.9 kDa AUS1 holoproenzyme, was recombinantly expressed in E. coli and purified to homogeneity. The enzyme shows only diphenolase activity, catalyzing the conversion of chalcones to aurones and was characterized by SDS-PAGE and shot-gun type nanoUHPLC-ESI-MS/MS. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This is an open access article under the CC BY license
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
