Journal
FEBS LETTERS
Volume 589, Issue 1, Pages 31-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.11.020
Keywords
Lipoic acid synthesis; Ferredoxin; Plastid; Apicomplexa; Protein interactions; Two-hybrid system
Funding
- Deutsche Forschungsgemeinschaft [Se622/4-2]
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The only known redox system in the apicoplast, a plastid-like organelle of apicomplexan parasites, is ferredoxin and ferredoxin-associated reductase. Ferredoxin donates electrons to different enzymes, presumably including lipoate synthase (LipA), which is essential for fatty acid biosynthesis. We recombinantly expressed and characterized LipA from the protozoan parasite Toxoplasma gondii, generated LipA-specific antibodies and confirmed the apicoplast localization of LipA. Electron transfer from ferredoxin to LipA would require direct protein-protein interaction. Such a robust interaction between the two proteins was demonstrated in both yeast and bacterial two-hybrid systems. Taken together, our results provide strong evidence for a role of ferredoxin as an electron donor to LipA. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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