Journal
FEBS LETTERS
Volume 588, Issue 21, Pages 3924-3931Publisher
WILEY
DOI: 10.1016/j.febslet.2014.09.004
Keywords
Dihydrofolate reductase; Folate; Oxidative stress; Quinonoid dihydropteridine reductase; Tetrahydrobiopterin
Funding
- KAKENHI from MEXT [21500305, 25460346]
- CREST from JST, NEXT Supported Program for the Strategic Research Foundation at Private Universities
- Tsuruoka City and Yamagata Prefecture
- Grants-in-Aid for Scientific Research [21500305, 25460346] Funding Source: KAKEN
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Quinonoid dihydropteridine reductase (QDPR) catalyzes the regeneration of tetrahydrobiopterin (BH4), a cofactor for monoamine synthesis, phenylalanine hydroxylation and nitric oxide production. Here, we produced and analyzed a transgenic Qdpr (/) mouse model. Unexpectedly, the BH4 contents in the Qdpr (/) mice were not decreased and even increased in some tissues, whereas those of the oxidized form dihydrobiopterin (BH2) were significantly increased. We demonstrated that unlike the wild-type mice, dihydrofolate reductase regenerated BH4 from BH2 in the mutants. Furthermore, we revealed wide alterations in folate-associated metabolism in the Qdpr (/) mice, which suggests an interconnection between folate and biopterin metabolism in the transgenic mouse model. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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