Journal
FEBS LETTERS
Volume 588, Issue 17, Pages 2957-2963Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.05.048
Keywords
Human mesenchymal stem cells; miR-140-5p; Bone morphogenic protein 2; Differentiation; Osteogenic lineage commitment
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science, and Technology [2012R1A1A3013555]
- Next-Generation BioGreen 21 Program - Rural Development Administration [PJ009097]
- Korean Health Technology R&D Projects - Ministry of Health and Welfare, Republic of Korea [A120476, A121957]
- National Research Foundation of Korea [2012R1A1A3013555] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Human mesenchymal stem cells (hMSCs) have self-renewal and differentiation capabilities but the regulatory mechanisms of MSC fate determination remain poorly understood. Here, we aimed to identify microRNAs enriched in hMSCs that modulate differentiation commitments. Microarray analysis revealed that miR-140-5p is commonly enriched in undifferentiated hMSCs from various tissue sources. Moreover, bioinformatic analysis and luciferase reporter assay validated that miR-140-5p directly represses bone morphogenic protein 2 (BMP2). Furthermore, blocking miR-140-5p in hMSCs increased the expression of BMP signaling components and critical regulators of osteogenic differentiation. We propose that miR-140-5p functionally inhibits osteogenic lineage commitment in undifferentiated hMSCs. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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