Journal
FEBS LETTERS
Volume 588, Issue 21, Pages 3862-3869Publisher
WILEY
DOI: 10.1016/j.febslet.2014.09.032
Keywords
Selective autophagy; Autophagosome; Nitrogen starvation; Yeast; Casein kinase 1; Vacuolar enzyme
Funding
- National Bio-Resource Project (NBRP) of the MEXT, Japan
- Next Generation World-Leading Researchers [HO220017]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [25111003, 25711005, 23000015]
- [pGEX-6P-Atg34]
- Grants-in-Aid for Scientific Research [25711005, 25111003] Funding Source: KAKEN
Ask authors/readers for more resources
In Saccharomyces cerevisiae, under nitrogen-starvation conditions, the a-mannosidase Ams1 is recognized by the autophagic receptor Atg34 and transported into the vacuole, where it functions as an active enzyme. In this study, we identified Hrr25 as the kinase that phosphorylates Atg34 under these conditions. Hrr25-mediated phosphorylation does not affect the interaction of Atg34 with Ams1, but instead promotes Atg34 binding to the adaptor protein Atg11, which recruits the autophagy machinery to the Ams1-Atg34 complex, resulting in activation of the vacuolar transport of Ams1. Our findings reveal the regulatory mechanism of a biosynthetic pathway mediated by the autophagy machinery. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available