Journal
FEBS LETTERS
Volume 588, Issue 4, Pages 632-640Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2014.01.009
Keywords
MicroRNA; MiR-155; MiR-455; Regulation of gene expression; Dendritic cell; Macrophage
Funding
- Deutsche Forschungsgemeinschaft [SFB 960, FOR855]
- European Research Council (ERC Grant) [242792]
- Bavarian Genome Research Network (BayGene)
- Bavarian Systems-Biology Network (BioSys-Net)
- European Union [201102]
- European Research Council (ERC) [242792] Funding Source: European Research Council (ERC)
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MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence of miR-155 on the expression levels of other miRNAs. For example, miR-455 is negatively regulated in miR-155-deficient cells possibly due to inhibition of the transcription factor C/EBPbeta by miR-155. Based on our comprehensive data sets, we propose a model of hierarchical miRNA expression dominated by miR-155 in DCs and macrophages. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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