Journal
FEBS LETTERS
Volume 587, Issue 9, Pages 1440-1445Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.03.027
Keywords
Thermosensitive transient receptor potential (TRP) ion channel; TRPV2; Human dendritic cell; Phagocytosis; RNA interference
Funding
- EU [FP7-REGPOT-2008-1/229920]
- [LP003/2011]
- [TAMOP 4.2.1./B-09/1/KONV-2010-0007]
- [TAMOP-4.2.2./A-11/1/KONV-2012-0025]
- [OTKA 78398]
- [OTKA 101761]
Ask authors/readers for more resources
Our goal was to investigate the effect of heat shock on human monocyte-derived dendritic cells (DCs) and to dissect the role of thermosensitive transient receptor potential (TRP) channels in the process. We provide evidence that a short heat shock challenge (43 degrees C) decreased the endocytotic activity of the DCs and that this effect could be alleviated by the RNAi-mediated knockdown of TRPV2 but, importantly, not by the pharmacological (antagonists) or molecular (RNAi) suppression of TRPV1 and TRPV4 activities/levels. Likewise, the heat shock-induced robust membrane currents were selectively and markedly inhibited by TRPV2 silencing whereas modulation of TRPV1 and TRPV4 activities, again, had no effect. These intriguing data introduce TRPV2-coupled signaling as a key player in mediating the cellular actions of heat shock on DCs. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available