Journal
FEBS LETTERS
Volume 588, Issue 2, Pages 206-212Publisher
WILEY
DOI: 10.1016/j.febslet.2013.11.024
Keywords
Disulfide; Engineering; Protein; Thermodynamics; Kinetics; Stability
Funding
- Wayne State University
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Improving the stability of proteins is an important goal in many biomedical and industrial applications. A logical approach is to emulate stabilizing molecular interactions found in nature. Disulfide bonds are covalent interactions that provide substantial stability to many proteins and conform to well-defined geometric conformations, thus making them appealing candidates in protein engineering efforts. Disulfide engineering is the directed design of novel disulfide bonds into target proteins. This important biotechnological tool has achieved considerable success in a wide range of applications, yet the rules that govern the stabilizing effects of disulfide bonds are not fully characterized. Contrary to expectations, many designed disulfide bonds have resulted in decreased stability of the modified protein. We review progress in disulfide engineering, with an emphasis on the issue of stability and computational methods that facilitate engineering efforts. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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