Journal
FEBS LETTERS
Volume 588, Issue 2, Pages 261-268Publisher
WILEY
DOI: 10.1016/j.febslet.2013.10.016
Keywords
Cell-free protein synthesis; Transcription; Translation; High throughput
Funding
- NIH [GM080376]
- Human Frontier Science Program postdoctoral fellowship
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The translation machinery is the engine of life. Extracting the cytoplasmic milieu from a cell affords a lysate capable of producing proteins in concentrations reaching to tens of micromolar. Such lysates, derivable from a variety of cells, allow the facile addition and subtraction of components that are directly or indirectly related to the translation machinery and/or the over-expressed protein. The flexible nature of such cell-free expression systems, when coupled with high throughput monitoring, can be especially suitable for protein engineering studies, allowing one to bypass multiple steps typically required using conventional in vivo protein expression. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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