Journal
FEBS LETTERS
Volume 587, Issue 24, Pages 3986-3994Publisher
WILEY
DOI: 10.1016/j.febslet.2013.10.034
Keywords
CD45; Galectin-3; Binding; Apoptosis; N-Glycans; O-Glycans
Funding
- National Natural Science Foundation of China [81301437]
- PUMC Youth Fund [2012J24]
- Fundamental Research Funds for the Central Universities of China [DWS201208]
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Galectin-3 has been reported to induce apoptosis of Jurkat cells through binding receptors such as CD45. CD45RABC is heavily O-glycosylated and N-glycosylated, while CD45RO is only N-glycosylated. In this study, no apoptosis induced by galectin-3 was detected in CD45RO-transfected cells, whereas apoptosis of CD45RABC-transfected cells was observed, implying that O-glycans on CD45 might play roles in galectin-3-induced apoptosis. O-Glycosylation inhibition assay further suggests the role of O-glycans on CD45 in regulation of galectin-3-induced apoptosis. We also found that deglycosylation at N327 of CD45RO resulted in increased binding to galectin-3 without affecting apoptosis, while deglycosylation at N36 or N109 of CD45RO enhanced galectin-3-induced apoptosis. These data demonstrate that galectin-3-induced apoptosis of Jurkat cells is regulated by both O-glycans and N-glycans on CD45. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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