Journal
FEBS LETTERS
Volume 587, Issue 22, Pages 3703-3708Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.09.037
Keywords
miR159; MYB33/65; amiRNAs; Efficacy; Gene silencing; miRNA abundance; Arabidopsis
Funding
- Australian Research Council [DP11013493]
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In plants, the silencing efficacy of microRNAs (miRNAs) is thought to be predominantly determined by the degree of complementarity to their target genes. Here, silencing efficacy was determined for Arabidopsis miR159 and four artificial miRNAs (amiRNAs) that all target MYB33/MYB65 with analogous complementarities. As determined through complementation of a loss-of-function mir159 mutant, the amiRNAs displayed highly variable efficacies, none of which was as strong as endogenous miR159. This was despite amiRNA expression levels being many fold-higher than miR159 in wild-type. The results highlight the variable nature of miRNA silencing efficacy in plants, where it appears that factors additional to complementarity strongly impact silencing. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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