Journal
FEBS LETTERS
Volume 587, Issue 15, Pages 2364-2370Publisher
WILEY
DOI: 10.1016/j.febslet.2013.06.002
Keywords
Algal biodiesel; Bioenergy; Triacylglycerol; Lipid droplet; Diacylglycerol acyltransferase; Chlamydomonas reinhardtii
Funding
- Japan Science and Technology Agency, PRESTO
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Algal triacylglycerol biosynthesis is of increasing interest for potential biodiesel production. A model microalga, Chlamydomonas, has multiple isoforms of diacylglycerol acyltransferase type 2 (DGTT) catalyzing the final step of triacylglycerol biosynthesis; however, the functions of the isoforms are poorly understood. Here, we performed heterologous complementation assay of Chlamydomonas DGTT1 to 4 in a yeast mutant defective in triacylglycerol biosynthesis. DGTT1, 2 and 3 but not 4 complemented the phenotype, including triacylglycerol levels. Interestingly, complementation by DGTT2 increased triacylglycerol content by 9-fold. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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