Journal
FEBS LETTERS
Volume 587, Issue 14, Pages 2099-2104Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2013.04.049
Keywords
BNC1; HSF2BP; Yeast two-hybrid; Spermatogenesis
Funding
- Ministry of Science and Technology of China [2012CB966702, 2013CB945001]
- National Natural Science Foundation of China [31271379, 31100984]
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Basonuclin (BNC1), a zinc finger transcriptional factor, is essential for mouse spermatogenesis. However, the regulatory mechanisms of BNC1 in spermatogenesis are poorly understood. In this study, we identified HSF2BP, a testis-specific binding protein of HSF2, as a binding partner of BNC1 by using yeast two-hybrid screening. HSF2BP could interact with and inhibit BNC1 transcriptional activity without affecting its expression level. Moreover, coexpression of HSF2BP with BNC1 resulted in a striking redistribution of BNC1 to the cytoplasm. These data suggest that HSF2BP may play a pivotal role in regulating BNC1 transcriptional activity and subcellular localization during spermatogenesis. Structured summary of protein interactions: Bnc1 physically interacts with HSF2BP by two hybrid (View interaction) Bnc1 and HSF2BP colocalize by fluorescence microscopy (View interaction) Bnc1 physically interacts with HSF2BP by anti tag coimmunoprecipitation (View Interaction: 1, 2) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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