Journal
FEBS LETTERS
Volume 586, Issue 4, Pages 442-447Publisher
WILEY
DOI: 10.1016/j.febslet.2012.01.027
Keywords
Bladder cancer; Cell cycle; Cyclin-dependent Kinase 4; microRNA; miR-195
Funding
- Key Lab of Multi-Organ Transplantation of Health Ministry and Zhejiang University Laboratory Animal Center
- National Natural Science Foundation of China [30973466, 30801370]
- Natural Science Foundation of Zhejiang Province [Z2090356]
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miRNAs are a class of small-noncoding RNAs capable of negatively regulating gene expression. Here, we found that miR-195 is down-regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role of miR-195 in bladder cancer, we conducted gain of function analysis by transfecting bladder cancer cell line T24 with chemically synthesized miR-195 mimic. We identified CDK4, an early G1 cell cycle regulator, as a novel target of miR-195. Selective overexpression of miR-195 could induce G1-phase arrest in T24 cells, and subsequently inhibit T24 cell growth. These findings indicate that miR-195 could be a potential tumor suppressor in bladder cancer. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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