Journal
FEBS LETTERS
Volume 586, Issue 21, Pages 3831-3839Publisher
WILEY
DOI: 10.1016/j.febslet.2012.08.023
Keywords
microRNA; Glioblastoma stem cell; Brain tumor; microRNA-125b; E2F2
Funding
- National Nature Science Foundation of China [81072065]
- Basic research projects of Qingdao Science and technology plan [12-1-4-8-(4)-jch]
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microRNAs (miRNAs) play important role in regulating cancer stem cell self-renewal and differentiation, but the expression prolife of miRNAs in glioma stem cells (GSCs) has not been addressed. Here, we found that CD133 positive GSCs possess a unique miRNAs profile compared to CD133 negative glioblastoma cells. miR-125b, as one of neuronal miRNAs, is the most significantly down-regulated miRNAs and overexpression of miR-125b inhibits the proliferation of CD133 positive GSCs and reduces the expression of stem marker. Furthermore, two binding sites for miR-125b are identified in the 3'UTR of E2F2 and overexpression of miR-125b in CD133 positive GSCs represses the endogenous level of E2F2 protein. This study demonstrated that miR-125b plays important roles in regulating the proliferation of GSCs by directly targeting E2F2. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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