Journal
FEBS LETTERS
Volume 586, Issue 23, Pages 4076-4081Publisher
WILEY
DOI: 10.1016/j.febslet.2012.10.009
Keywords
SIRT5; Urate oxidase; Mouse; Liver; Mitochondria
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Ministry of Health, Labor, Welfare, Japan
- CREST grant from the Japan Science and Technology Agency
- Kyoto University Global COE Program Center for Frontier Medicine
- Grants-in-Aid for Scientific Research [23791026] Funding Source: KAKEN
Ask authors/readers for more resources
We identified urate oxidase (UOX) as a target of SIRT5 by comparing mitochondrial proteins in livers of SIRT5-overexpressing transgenic (SIRT5 Tg) and wild-type mice by using two-dimensional electrophoresis. Acetylation levels of UOX in liver of SIRT5 Tg mice were approximately half of those in wild-type mice, and UOX activity was significantly increased. In vitro-synthesized UOX protein was acetylated when incubated with mitochondria from wild-type mice liver but the levels were less when incubated with those from SIRT5 Tg mice liver. These results suggest that SIRT5 activates UOX through deacetylation in mouse liver mitochondria.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available