Journal
FEBS LETTERS
Volume 586, Issue 16, Pages 2494-2499Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.06.012
Keywords
D-Galacturonic acid transport; Yeast; Pectins; Channel-type transport; Uronic acid; Bioethanol
Funding
- Institute for Scientific and Technological Research
- KU Leuven research fund [CREA/07/012]
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D-Galacturonic acid is a major component of pectins but cannot be metabolized by Saccharomyces cerevisiae. It is assumed not to be taken up. We show that yeast displays surprisingly rapid low-affinity uptake of D-galacturonic acid, strongly increasing with decreasing extracellular pH and without saturation up to 1.5 M. There was no intracellular concentration above the extracellular level and transport was reversible. Among more than 160 single and multiple deletion mutants in channels and transporters, no strain was affected in D-galacturonic acid uptake. The uptake was not inhibited by any compound tested as candidate competitive inhibitor, including D-glucuronic acid, which was also transported. The characteristics of D-galacturonic acid uptake are consistent with involvement of a channel-type system, probably encoded by multiple genes. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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