Journal
FEBS LETTERS
Volume 586, Issue 7, Pages 1022-1026Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.02.045
Keywords
Toll-like receptor; NLRP3; NF-kappa B; Macrophage
Funding
- National Natural Science Foundation of China [81172813, 31000407]
- Taishan Scholar Program of Shandong Province
- Shandong Provincial Nature Science Foundation for Distinguished Young Scholars [JQ201120]
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NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1 beta. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-kappa B dependent manner. Furthermore, the corresponding NF-kappa B binding sites (nt -1303 to -1292 and -1238 to -1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-kappa B binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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