Journal
FEBS LETTERS
Volume 587, Issue 2, Pages 231-237Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2012.12.002
Keywords
Apoptosis; Burkitt's lymphoma; Keratan sulfate; PAPST; Radiation
Funding
- KAKENHI [24591856]
- Grants-in-Aid for Scientific Research [24591856] Funding Source: KAKEN
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This study focuses on clarifying the contribution of sulfation to radiation-induced apoptosis in human Burkitt's lymphoma cell lines, using 3'-phosphoadenosine 5'-phosphosulfate transporters (PAPSTs). Overexpression of PAPST1 or PAPST2 reduced radiation-induced apoptosis in Namalwa cells, whereas the repression of PAPST1 expression enhanced apoptosis. Inhibition of PAPST slightly decreased keratan sulfate (KS) expression, so that depletion of KS significantly increased radiation-induced apoptosis. In addition, the repression of all three N-acetylglucosamine-6-O-sulfotransferases (CHST2, CHST6, and CHST7) increased apoptosis. In contrast, PAPST1 expression promoted the phosphorylation of p38 MAPK and Akt in irradiated Namalwa cells. These findings suggest that 6-O-sulfation of GlcNAc residues in KS reduces radiation-induced apoptosis of human Burkitt's lymphoma cells. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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